Lab 7 Open Analysis, Overview and Reflection

As this week comes to a close, we have all completed lab 7 and our on our way with the DNA replication process. We are currently taking samples of our own DNA, denaturing it, replicating it, and allowing it to undergo a polymerase chain reaction so that we can amplify specific DNA sequences. We will then be putting it through electrophoresis in order to determine our blood types. We have completed lab one of a series of four and today was simply gathering our own DNA and synthesizing short oligonucleotides. Theses are primers that are used to prime the replication of the target DNA by DNA polymerase. Two cycles of PCR where carried out so that we produced four sets of double stranded DNA. Overall the lab was a success and very interesting. I as well as my fellow students look foreword to the labs that await and the rest of the DNA sequencing that is yet to come. Please leave any additional comments, thoughts or insight pertaining to this lab.